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Table 2 Summary of 3-D culture studies with follicles from human, primate and large domestic animal species

From: Three-dimensional in vitro follicle growth: overview of culture models, biomaterials, design parameters and future directions

Author Year System
Description
Species Culture
Period
Initial
Follicle
Diameter
Final
Diameter
Survival
Rate
Antrum
Formation
Observations/Conclusions
Amorim
et al.
[72]
2009 Alginate (ALG)1% Human 7 days 34-52 μm 44--70 μm 90% NA Alginate culture system supported growth of Isolated follicles from frozen-thawed ovary
Xu
et al.
[67]
2009 Alginate 0.5%
Matrigel embedded
Human 30 days ~175 μm 715 μm NA 75% Both 3-D systems supported growth of isolated human follicles
Xu
et al.
[48]
2009 Alginate (ALG) 0.25% versus 0.5% Rhesus monkey 30 days 100-300 μm 20 vs 78% 60 vs 78% Yes Higher ALG better survival and growth. LH addition with FSH negative effect on survival and P4 secretion
Itoh
et al.
[104]
2002 Collagen gel Cow 13 days 145-170 μm 304 μm NA Yes Serum-free culture. Insulin, FSH and LH together induced earlier antrum formation
Abir
et al.
[51]
2001 Collagen gel Human 24 hours 35-45 μm 70 μm NA NA Collagen matrix supported growth of fully isolated follicles but not tissue slice with partially isolated follicles
Hovatta
et al.
[49]
1999 In situ and partially isolated follicles
Millicell + Matrigel
Human ~28 days
total
NA NA NA No Tissue slices better less oocyte extrusion than collagenese isolated. Four weeks to reach secondary stage
Yamamoto
et al.
[105]
1999 Collagen gel Cow 14 days 500-700 μm NA 37% Yes MII 27%, 42% fertilization, 4% blastocyst
One live birth.
Hiraoi
et al.
[106]
1994 Collagen gel Pig 16 days 220-300 μm NA NA Yes 40% MII formation in oocytes ≥110 μm
No MII from oocytes <110 μm. Oocytes capable of being fertilized
Roy and Treacy
[107]
1993 Agar Human 5 days 90-220 μm NA NA Yes FSH induced antrum formation, hormone secretion. No FSH, no E2 secretion
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