Table 2 Summary of 3-D culture studies with follicles from human, primate and large domestic animal species
Author | Year | System Description | Species | Culture Period | Initial Follicle Diameter | Final Diameter | Survival Rate | Antrum Formation | Observations/Conclusions |
|---|---|---|---|---|---|---|---|---|---|
Amorim et al. [72] | 2009 | Alginate (ALG)1% | Human | 7 days | 34-52 μm | 44--70 μm | 90% | NA | Alginate culture system supported growth of Isolated follicles from frozen-thawed ovary |
Xu et al. [67] | 2009 | Alginate 0.5% Matrigel embedded | Human | 30 days | ~175 μm | 715 μm | NA | 75% | Both 3-D systems supported growth of isolated human follicles |
Xu et al. [48] | 2009 | Alginate (ALG) 0.25% versus 0.5% | Rhesus monkey | 30 days | 100-300 μm | 20 vs 78% | 60 vs 78% | Yes | Higher ALG better survival and growth. LH addition with FSH negative effect on survival and P4 secretion |
Itoh et al. [104] | 2002 | Collagen gel | Cow | 13 days | 145-170 μm | 304 μm | NA | Yes | Serum-free culture. Insulin, FSH and LH together induced earlier antrum formation |
Abir et al. [51] | 2001 | Collagen gel | Human | 24 hours | 35-45 μm | 70 μm | NA | NA | Collagen matrix supported growth of fully isolated follicles but not tissue slice with partially isolated follicles |
Hovatta et al. [49] | 1999 | In situ and partially isolated follicles Millicell + Matrigel | Human | ~28 days total | NA | NA | NA | No | Tissue slices better less oocyte extrusion than collagenese isolated. Four weeks to reach secondary stage |
Yamamoto et al. [105] | 1999 | Collagen gel | Cow | 14 days | 500-700 μm | NA | 37% | Yes | MII 27%, 42% fertilization, 4% blastocyst One live birth. |
Hiraoi et al. [106] | 1994 | Collagen gel | Pig | 16 days | 220-300 μm | NA | NA | Yes | 40% MII formation in oocytes ≥110 μm No MII from oocytes <110 μm. Oocytes capable of being fertilized |
Roy and Treacy [107] | 1993 | Agar | Human | 5 days | 90-220 μm | NA | NA | Yes | FSH induced antrum formation, hormone secretion. No FSH, no E2 secretion |