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Table 1 Studies examining matrices for 3-D culture of pre-antral ovarian follicles from rodents

From: Three-dimensional in vitro follicle growth: overview of culture models, biomaterials, design parameters and future directions

Author Year System
Description
Species Culture
Period
Initial Follicle
Diameter
Survival rate
(%)
GVBD
(%)
MII Formation
(%)
Additional Outcomes/
Observations
Jin
et al.
[102]
2010 In situ culture followed by encapsulation of follicles in 0.25% ALG-alginate or FA-fibrin alginate Mouse In situ:
4 days
Alginate: 12 days
NA Alginate: 69.6%
FA: 75%
Alginate: 75%
FA: 86%
(from follicles with antrum formation)
Alginate: 61.3 ± 2.4
FA: 88 ± 8.7
(from oocytes undergoing GVBD)
Formation of 2 cell embryos
Alginate: 33%
FA: 54 ± %
Xu
et al.
[73]
2009 Fresh follicles (Foll) and cryopreserved (Cryo-Foll) and crypreserved ovarian tissue (Cryo-OV)
ALG-alginate (0.25%)
Mouse 12 days 100-130 μm Fresh-Foll 78%
Cryo-Foll 74%
Cryo-OV 72%
Fresh-Foll: 83%
Cryo-Foll 69%
Cryo-OV: 92%
Fresh-Foll:59%
Cryo-Foll: 64%
Cryo-Ov 68%
Gap junction protein, connexin expression also studied-down regulated after cryopreservation
Shikanov
et al.
[103]
2009 ALG (0.25%)
FA fibrin alginate
Mouse 12 days 100-130 μm ALG:78%
FA:77-81%
ALG:88%
FA: 72-88%
ALG:67%
FA:75-82%
NA
West
et al.
[45]
2007 ALG (0.7%, 1.5%, 3%) Mouse 8-12 days 100-130 μm
150-180 μm
100-130 μm 15-42%
150-180 um 83-91%
100-130 μm 31-66%
150-180 um 46-91%
NA Low matrix stiffness increase growth, antrum, GVBD and E2
Kreeger
et al.
[75]
2006 Tested alginate with ECM
2 versus multi-layer follicles
ALG alginate alone (1.5%)
CI collagen I
FN fibronectin
RGD peptides
CIV collagen IV
LN laminin
Mouse 8 days 100-130 μm
versus
150-180 μm
ALG 64% vs 69%
CI 65% vs 67%
FN 70% vs 72%
RGD 72% vs 62%
CIV 72% vs 48%
LN 63% vs 61%
ALG 12% vs 38%
CI 18% vs 25%
FN 23% vs 17%
RGD 13% vs 36%
CIV 20% vs 36%
LN 29% vs 11%
ALG 40%
CI 44%
FN 71%
RGD 65%
CIV 50%
LN 71%
*Multi-layer follicles
Transition to secondary Follicle promoted by CI and RGD
MII formation promoted by:
FN, RGD, LN
Xu
et al.
[39]
2006 ALG (1.5%) Mouse 8 days 150-180 μm 93% 82% 71% Live births of pups
Xu
et al.
[46]
2006 ALG (0.25%, 0.5%,1%.1.5%) Mouse 12 days 100-130 μm 74-85% 78-88% 56-67% Decreasing 2-cell and blast with increasing % ALG
Heise
et al.
[36]
2005 ALG (1%)
Encapsulation with or without FSH in gel medium
Rat 72 hrs 150-160 um NA NA NA Inclusion FSH with ALG and culture medium, Follicle diameter increased by 33%
Mousset-Simeon
et al.
[26]
2005 Microdrops under oil
Agar Millicell-CM membrane insert
Mouse 12 days 100-130 μm Microdrops 72%
Membrane 46%
Agar 30%
Microdrops 63%
Membrane 33%
Agar 26%
Microdrops 53%, Membrane 56% Agar 13% 2-D Microdrop high survival.
Maturation rate similar to 3-D on membrane
Kreeger
et al.
[71]
2005 Compared effects two versus multi-layered follicles
ALG(1.5%)-Collagen I matrix FSH 5-50 mIU/ml
Mouse 8 days 100-130 μm
150-180 μm
66-77%
30-72%
21-27%
9-43%
Multi-layer follicles
40-78%
Hormone secretion (E2 and progesterone) in multi-layer follicles FSH dependent
Loret de Mola
et al.
[63]
2004 Collagen treated membrane Collagen gel encapsulation Mouse 10 days 118 μm Membrane 55%
Collagen gel 15%
NA Membrane 17%
Collagen gel 19%
*Based on number of recovered eggs
Follicle size larger in collagen gel but maturation and rate of 2-cell formation not enhanced
Adam
et al.
[25]
2004 Microdrops under oil
Millicell-CM membrane insert
Mouse 6 days 150-174 μm
175-200 μm
Microdrops 77%
Membrane 83%
NA Membrane 79% Membrane insert
Fert rate 75%, blast rate 48%
Pangas
et al.
[70]
2003 Alginate Mouse 10 days 82 μm 68% NA 40% TEM indicate follicles in ALG maintained ultrastructure
Gomes
et al.
[62]
1999 Collagen gel encapsulation Mouse 6 days 135 μm NA NA NA Follicle volume and response to FSH increased with 3-D culture in collagen
Nayudu
et al.
[54]
1992 Millicell-CM membrane insert Mouse 6-7 days
3-5 days
125-150 μm
150-180 μm
NA NA NA FSH stimulated growth, antrum formation, E2 dose response to FSH levels
Torrance
et al.
[61]
1989 Collagen gel encapsulation Mouse 14 days 20-95 μm 36% NA NA Growth to multi-laminar stage but no antrum formation
  1. NA: Not available
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