Table 1 Studies examining matrices for 3-D culture of pre-antral ovarian follicles from rodents
Author | Year | System Description | Species | Culture Period | Initial Follicle Diameter | Survival rate (%) | GVBD (%) | MII Formation (%) | Additional Outcomes/ Observations |
|---|---|---|---|---|---|---|---|---|---|
Jin et al. [102] | 2010 | In situ culture followed by encapsulation of follicles in 0.25% ALG-alginate or FA-fibrin alginate | Mouse | In situ: 4 days Alginate: 12 days | NA | Alginate: 69.6% FA: 75% | Alginate: 75% FA: 86% (from follicles with antrum formation) | Alginate: 61.3 ± 2.4 FA: 88 ± 8.7 (from oocytes undergoing GVBD) | Formation of 2 cell embryos Alginate: 33% FA: 54 ± % |
Xu et al. [73] | 2009 | Fresh follicles (Foll) and cryopreserved (Cryo-Foll) and crypreserved ovarian tissue (Cryo-OV) ALG-alginate (0.25%) | Mouse | 12 days | 100-130 μm | Fresh-Foll 78% Cryo-Foll 74% Cryo-OV 72% | Fresh-Foll: 83% Cryo-Foll 69% Cryo-OV: 92% | Fresh-Foll:59% Cryo-Foll: 64% Cryo-Ov 68% | Gap junction protein, connexin expression also studied-down regulated after cryopreservation |
Shikanov et al. [103] | 2009 | ALG (0.25%) FA fibrin alginate | Mouse | 12 days | 100-130 μm | ALG:78% FA:77-81% | ALG:88% FA: 72-88% | ALG:67% FA:75-82% | NA |
West et al. [45] | 2007 | ALG (0.7%, 1.5%, 3%) | Mouse | 8-12 days | 100-130 μm 150-180 μm | 100-130 μm 15-42% 150-180 um 83-91% | 100-130 μm 31-66% 150-180 um 46-91% | NA | Low matrix stiffness increase growth, antrum, GVBD and E2 |
Kreeger et al. [75] | 2006 | Tested alginate with ECM 2 versus multi-layer follicles ALG alginate alone (1.5%) CI collagen I FN fibronectin RGD peptides CIV collagen IV LN laminin | Mouse | 8 days | 100-130 μm versus 150-180 μm | ALG 64% vs 69% CI 65% vs 67% FN 70% vs 72% RGD 72% vs 62% CIV 72% vs 48% LN 63% vs 61% | ALG 12% vs 38% CI 18% vs 25% FN 23% vs 17% RGD 13% vs 36% CIV 20% vs 36% LN 29% vs 11% | ALG 40% CI 44% FN 71% RGD 65% CIV 50% LN 71% *Multi-layer follicles | Transition to secondary Follicle promoted by CI and RGD MII formation promoted by: FN, RGD, LN |
Xu et al. [39] | 2006 | ALG (1.5%) | Mouse | 8 days | 150-180 μm | 93% | 82% | 71% | Live births of pups |
Xu et al. [46] | 2006 | ALG (0.25%, 0.5%,1%.1.5%) | Mouse | 12 days | 100-130 μm | 74-85% | 78-88% | 56-67% | Decreasing 2-cell and blast with increasing % ALG |
Heise et al. [36] | 2005 | ALG (1%) Encapsulation with or without FSH in gel medium | Rat | 72 hrs | 150-160 um | NA | NA | NA | Inclusion FSH with ALG and culture medium, Follicle diameter increased by 33% |
Mousset-Simeon et al. [26] | 2005 | Microdrops under oil Agar Millicell-CM membrane insert | Mouse | 12 days | 100-130 μm | Microdrops 72% Membrane 46% Agar 30% | Microdrops 63% Membrane 33% Agar 26% | Microdrops 53%, Membrane 56% Agar 13% | 2-D Microdrop high survival. Maturation rate similar to 3-D on membrane |
Kreeger et al. [71] | 2005 | Compared effects two versus multi-layered follicles ALG(1.5%)-Collagen I matrix FSH 5-50 mIU/ml | Mouse | 8 days | 100-130 μm 150-180 μm | 66-77% 30-72% | 21-27% 9-43% | Multi-layer follicles 40-78% | Hormone secretion (E2 and progesterone) in multi-layer follicles FSH dependent |
Loret de Mola et al. [63] | 2004 | Collagen treated membrane Collagen gel encapsulation | Mouse | 10 days | 118 μm | Membrane 55% Collagen gel 15% | NA | Membrane 17% Collagen gel 19% *Based on number of recovered eggs | Follicle size larger in collagen gel but maturation and rate of 2-cell formation not enhanced |
Adam et al. [25] | 2004 | Microdrops under oil Millicell-CM membrane insert | Mouse | 6 days | 150-174 μm 175-200 μm | Microdrops 77% Membrane 83% | NA | Membrane 79% | Membrane insert Fert rate 75%, blast rate 48% |
Pangas et al. [70] | 2003 | Alginate | Mouse | 10 days | 82 μm | 68% | NA | 40% | TEM indicate follicles in ALG maintained ultrastructure |
Gomes et al. [62] | 1999 | Collagen gel encapsulation | Mouse | 6 days | 135 μm | NA | NA | NA | Follicle volume and response to FSH increased with 3-D culture in collagen |
Nayudu et al. [54] | 1992 | Millicell-CM membrane insert | Mouse | 6-7 days 3-5 days | 125-150 μm 150-180 μm | NA | NA | NA | FSH stimulated growth, antrum formation, E2 dose response to FSH levels |
Torrance et al. [61] | 1989 | Collagen gel encapsulation | Mouse | 14 days | 20-95 μm | 36% | NA | NA | Growth to multi-laminar stage but no antrum formation |