'ZP domain' of human zona pellucida glycoprotein-1 binds to human spermatozoa and induces acrosomal exocytosis

Table 2 Induction of acrosome reaction by baculovirus-expressed recombinant human ZP1_273-551aa in capacitated human spermatozoa

Treatment	Percent stimulation of acrosome reaction^a (Mean ± SEM)	Statistical significance^b
Fetuin (10 μg/ml)	7.6 ± 1.8
ZP1 (10 μg/ml)	33.2 ± 2.9	p = 0.0065*
ZP2 (10 μg/ml)	10.9 ± 2.4	p = 0.6085
ZP3 (10 μg/ml)	42.3 ± 3.2	p = 0.0023*
ZP1_273-551aa
500 ng/ml	17.2 ± 1.6	p = 0.0391*
1 μg/ml	30.1 ± 0.6	p = 0.0078*
2 μg/ml	58.2 ± 1.7	p = 0.0002*
5 μg/ml	40.5 ± 0.6	p = 0.0007*
10 μg/ml	41.6 ± 1.7	p = 0.0016 *

^aPercent stimulation of acrosomal exocytosis was calculated as stated in Methods. Values represent mean ± SEM of at least four independent experiments.
^bStatistical significance with respect to the acrosome reaction induced by Fetuin was calculated by one way ANOVA followed by Newman-Keuls Multiple Comparison Test.
*Values are statistically significant.

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