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Figure 5 | Reproductive Biology and Endocrinology

Figure 5

From: Differential genome-wide gene expression profiling of bovine largest and second-largest follicles: identification of genes associated with growth of dominant follicles

Figure 5

Localization of AMH, CYP19 and PlGF mRNA in healthy and atretic follicles. These genes were expressed more in F1 than in F2 in QPCR analysis. (A, C, E, G, I and K) DIG-labeled anti-sense cRNA probes were used. (B, D, F, H, J and L) DIG-labeled sense cRNA probes were used. Seven-micrometer sections of bovine follicles were hybridized with each probe. AMH (A, B, C and D) and CYP19 (E, F, G and H) mRNA was localized in granulosa cells (GC) of healthy as well as atretic follicles. PlGF mRNA (I, J, K and L) was found in GC and theca layer of healthy follicle but not atretic follicle. Scale bar = 20 μm.

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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