Figure 3

Western blot analysis of AQP9 expression. Expression of AQP9 assessed by Western blotting in the endometrium of porcine uterus on Days 2-4, 10-12, 14-16 and 18-20 of estrous cycle (A) as well as on Days 14-16 and 30-32 of pregnancy (B). Densitometric analyses of AQP9 protein levels normalized against β-actin (A and B). The data are summarized for endometrial expression of AQP9 during studied stages of the cycle and pregnancy (A and B). Comparison of AQP9 expression determined by Western blotting in porcine endometrium between two stages of the cycle and two periods of pregnancy (C). Immunoblotting for AQP5 protein (D) in the uterus. The blots were probed with anti-AQP5 (Lane 2) or anti-AQP5 preadsorbed with the excess synthetic peptide (Lane 1). RT-PCR was performed on total RNA (E) from uteral tissue. Values are the mean ± SEM (n = 5). ^a,bMeans with different superscripts are significantly different.