Western blot analysis of AQP1 expression. Expression of AQP1 assessed by Western blotting in the endometrium and myometrium of porcine uterus on Days 2-4 (A), 10-12 (B), 14-16 (C) and 18-20 (D) of estrous cycle as well as on Days 14-16 (E) and 30-32 (F) of pregnancy. Densitometric analyses of AQP1 protein levels normalized against β-actin (A-F). The data are summarized in (G) for endometrial and myometrial (H) expression of AQP1 during studied stages of the cycle. Comparison of AQP1 expression determined by Western blotting in porcine endometrium (I) and myometrium (J), between two stages of the cycle and two periods of pregnancy. Immunoblotting for AQP1 protein (K) in the uterus. The blots were probed with anti-AQP1 (Lane 2) or anti-AQP1 preadsorbed with the excess synthetic peptide (Lane 1). RT-PCR was performed on total RNA (L) from uteral tissue. Values are the mean ± SEM (n = 5). a,bMeans with different superscripts are significantly different.