Figure 5
From: Autocrine regulation of human sperm motility by tachykinins
Tachykinin receptor-selective antagonists inhibit the effect of phosphoramidon on sperm motility. The effects of phosphoramidon (1 μM, 60 min incubation) on human sperm progressive motility (grade a+b sperm) were analyzed in the presence of SR140333 (NK1 antagonist, 10 nM), SR48968 (NK2 antagonist, 10 nM), SR142801 (NK3 antagonist, 10 nM), a combination of the three antagonists, or the antagonist solvent. Motility analysis was performed manually following WHO guidelines. Bars are means with SEM of 6-8 different experiments and represent percentage changes in motility in samples treated with phosphoramidon relative to the value observed at the same time in phosphoramidon solvent-treated paired controls. *P < 0.05, significant difference vs. response to phosphoramidon.