Figure 2

Immunofluorescent localization of tachykinins in human sperm. (A) Immunofluorescence and corresponding differential interference contrast images of sperm cells stained with primary antibodies against substance P (SP), neurokinin A (NKA) and neurokinin B (NKB) showing specific localizations for each peptide. (B) Immunofluorescence and corresponding phase contrast images of sperm cells stained with a primary antibody against hemokinin-1 (HK-1) in the absence (a) and in the presence (b) of immunogenic peptide. For each tachykinin, experiments were performed at least six times with similar results. Scale bar, 10 μM.