Figure 1
From: Autocrine regulation of human sperm motility by tachykinins
Gene expression of tachykinin precursors, neprilysin ( MME ) and neprilysin-2 ( MMEL1 ) in human sperm (SPZ) and testis (TES). In human sperm, the specific bands corresponding to TAC1 γ and δ isoforms, TAC4 and MME were only detected after cDNA reamplification. Acrosin (ACR) was present in sperm and testis cDNA while CD4 was only detected in testis. (+), positive control showing the expression of all genes in a pool of cDNAs from twenty different human tissues. (-), negative control with no RNA in the reverse transcriptase reaction; M, molecular size standards. The figure is representative of results in 6 pools of sperm samples from 8 different donors.