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Figure 4 | Reproductive Biology and Endocrinology

Figure 4

From: Expression of COX-1 and COX-2 in the endometrium of cyclic, pregnant and in a model of pseudopregnant rats and their regulation by sex steroids

Figure 4

The expression of COX-1 (4A) and COX-2 proteins (4C) in rat endometrium during estrous cycle. Total endometrial proteins were collected at indicated days from polyoestrus cycling rats at each stage of the estrous cycle (diestrus, proestrus, estrus and metestrus) and subjected to western blots analysis of COX-1 and COX-2 content. Representative blots are shown. Histograms show densitometric analysis of COX-1 and COX-2 normalized to β-actin to correct for protein loading (4B&4D). Data represents the mean ± SEM of 3-4 independent experiments. Concentration of PGE2 and PGF2 α metabolites in serum of polyoestrus cycling rats at different stages of the estrous cycle (diestrus, proestrus, estrus and metestrus) (4E&4F). Serum samples were collected at indicated stage of estrous cycle and the metabolites were measured using a high sensitivity enzyme immunoassay (EIA) kit. Data represents the mean ± SEM of four independent experiments. Columns with different superscripts are significantly different (p < 0.05).

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