Figure 3
The expression of COX-1 (3A) and COX-2 proteins (3C) in rat endometrium during pseudopregnancy. Total endometrial proteins were collected at indicated days of pseudopregnancy and were analysed by western blotting. Representative blots are shown. Histograms show densitometric analysis of COX-1 and COX-2 normalized to β-actin to correct for protein loading (3B&3D). Data represents the mean ± SEM of 3-4 four independent experiments. Concentration of PGE2 and PGF2α metabolites in serum from pseudopregnant rat (3E&3F). Serum samples were collected at indicated days of peudopregnancy and the metabolites were measured using a high sensitivity enzyme immunoassay (EIA) kit. Data represents the mean ± SEM of four independent experiments. Columns with different superscripts are significantly different (p < 0.05).