Table 1 Construction of Gal4 fusions of mouse genes for yeast two-hybrid assay
Construction | Nucleotide accesion numbers in GenBank | Nucleotides | Primers used in PCR | Designed Restrictive Enzymes |
|---|---|---|---|---|
pGADT7-mCRA | AF359382 | 73-1431 | 5'-CGGGATCCAGATGATTTCTTCAAAGCCC AGAC-3' 5'-CCGCTCGAG TCAAACCTGTTCAGGAGCAGCTT-3' | BamH I Xho I |
pGADT7-mTCRA | AF359382 | 223-1431 | 5'-CGGGATCCACGGGAATTCGTCTCTAGATATAA-3' 5'-CCGCTCGAGTCAAACCTGTTCAGGAGCAGCTT-3' | BamH I Xho I |
pGADT7-mCRB | AF359382 | 1443-2039 | 5'-CGGAATTCGCACTAGCAACAAGCGAAGCAGG-3' 5'-CCGCTCGAGTTAAGGTTCTGCTCTGCGGACA-3' | EcoR I Xho I |
pGBKT7-mAKAP3 | NM_009650 | 292-2883 | 5'-CGGAATTCATGGCGGATAGGGTTGACTG-3' 5'-ACGTCGACCAGGTTTGCCATCAGCCAGTCC-3' | EcoR I Sal I |
pGBKT7-mAKAP4 | NM_009651 | 148-2664 | 5'-CGGAATTCATG TCTGATGACA TTGACTGGT-3' 5'-ACGTCGACCAGGTTAGCGAGAAGCCAGTCC-3' | EcoR I Sal I |
pGBKT7-ropporin | AF178531 | 1-636 | 5'-CATGCCATGGGACCTCAGACAGACAAGCAAG -3' 5'-CGGAATTCTTATTCCAGCCGAACCCTAGGGT-3' | Nco I EcoR I |