Figure 9

Interactions between various CABYR constructs and AKAP3, AKAP4 or ropporin in the yeast two-hybrid system. Yeast strain AH109 was co-transformed with pGADT7-CRA, pGADT7-TCRA or pGADT7-CRB and pGBKT7-AKAP3, pGBKT7-AKAP3 or pGBKT7-ropporin (pGADT7-T, while pGBKT7-53 served as a positive control and pGADT7-T and pGBKT7-Lam served as a negative control). The ability of the transformants to grow on selective SD/-Leu/-Trp plates (Fig. 11 A, C, E), SD/-Ade/-His/-Leu/-Trp plates (Fig. 11 B, D), or SD/-His/-Leu/-Trp (Fig. 11 F) was analyzed. A, B. Interaction between CABYR and AKAP3: 1, CR-A+AKAP3; 2, TCR-A+AKAP3; 3, CR-B +AKAP3; 4, p53 + Large T (positive control); 5, p53 + Lam C (negative control). While CABYR containing the RII domain showed signals equivalent to the positive control, the construct lacking the RII domain showed the signal equivalent to the negative control. C, D. Interaction between CABYR and AKAP4: 1, CR-A+AKAP4; 2, TCR-A+AKAP4; 3, CR-B +AKAP4; 4, p53 + Large T (positive control); 5, p53 + Lam C (negative control). The results are very similar to that of the interaction between CABYR and AKAP3. E, F. The analysis of interaction between CABYR and ropporin: 1, CR-A +ropporin; 2, TCR-A+ ropporin; 3, CR-B+ ropporin; 4, p53 + Large T (positive control); 5, p53 + Lam C (negative control). There is a weak interaction between CABYR-A, as well as truncated CABYR-A, and ropporin but not between CABYR-B and ropporin.