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Figure 5 | Reproductive Biology and Endocrinology

Figure 5

From: CABYR isoforms expressed in late steps of spermiogenesis bind with AKAPs and ropporin in mouse sperm fibrous sheath

Figure 5

Immunofluorescence and immunoelectron microscopic localization of mouse CABYR-A to the FS of principal piece on sperm flagellum. A, C: Phase contrast images; B, D: Indirect immunofluorescence staining using anti-CABYR-A serum (B) or pre-immune serum (D). Intense fluorescent staining of mouse CABYR-A using the anti-CABYR-A antiserum is restricted to the principal piece of all mouse sperm flagellum in B (×400). Pre-immune serum did not stain the spermatozoa (D ×400). E, F: immunoelectron microscopic localization with guinea pig anti-CABYR-A antibody. Most of the gold particles were located over the fibrous sheath (fs), including the surface of the longitudinal columns (lc) and ribs (r). Occasional gold particles were present at the surface of the outer dense fibers (odf). CABYR-A was not detected in the axoneme (ax) or the matrix (interior) of the outer dense fibers. G. Pre-immune serum controls with sperm in longitudinal and oblique cross sections show very few gold particles. Bar: 0.1 μm.

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