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Figure 3 | Reproductive Biology and Endocrinology

Figure 3

From: CABYR isoforms expressed in late steps of spermiogenesis bind with AKAPs and ropporin in mouse sperm fibrous sheath

Figure 3

CABYR-A only variant can be co-immunoprecipitated with CABYR-B containing variants. A, B: 2-D Western blots of proteins immunoprecipitated using anti-CABYR-A polyclonal antibody (A) or pre-immune serum (B) and probed by anti-CABYR-A primary antibody and anti-guinea pig secondary antibody. Heavy and light chain reactive spots are noted in pre-immune controls (B & D). IP with anti-CABYR-A brought down soluble forms of CABYR mainly including 80 and 50 kDa isoforms. The location of the 80 kDa CABYR-A only isoform is indicated by an arrow (A). The 80 and 50 kDa isoforms of mouse CABYR appear to be the most soluble and readily extracted. C, D: 2-D Western blots of mouse sperm immunoprecipitate using anti-CABYR-B polyclonal antibody (C) or pre-immune serum (D) probed by anti-CABYR-A polyclonal antibody. The 80 kDa spot indicated by an arrow (C) is a CABYR-A only variant and is co-immunoprecipitated with CABYR-B, demonstrating that the 80 kDa CABYR-A only variant complexes with CABYR-B containing variants.

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