Figure 10

α-Galactosidase assay and summary of the interactions. A: α-Galactosidase assay. Quantitative data are plotted with error bars (Standard Deviation) corresponding to three or four separate experiments. The α-galactosidase activity of the transformant bearing CR-A+AKAP3 is much higher than that of P53 and SV40 large T, while the activity of the transformant bearing CR-A+AKAP4 is comparable to that of P53 and SV40 large T. TCR-A+AKAP3 and TCR-A+AKAP4 have very low α-galactosidase activities, similar to the p53+Lam C (negative control). CR-B+AKAP3, CR-B+AKAP4, CR-A+ropporin and TCR-A+ropporin show much lower α-galactosidase activities than P53 and SV40 large T (positive control) but are significantly higher than p53+Lam C (negative control), suggesting that there may be some degree of weak interaction between these pairs of proteins. CR-B+ropporin also show very low α-galactosidase activity similar to the p53+Lam C (negative control). B: Summary of the interactions between segments of CABYR and AKAP3/AKAP4/Ropporin.