Figure 4

In situ hybridization analysis for cell-specific expression. Frozen sections of normal and cancerous hen ovaries were subjected to in situ hybridization analysis against sense and/or antisense probes of CTSB, CTSC, and CTSS. There was no mRNA expression detected in the normal and cancerous ovaries hybridized with sense probes of CTSB, CTSC, or CTSS. A low level cell-specific localization of CTSB, CTSC, or CTSS mRNA was detected in normal ovaries. CTSB mRNA was strongly expressed in the nodule area of cancerous hen ovaries. CTSC and CTSS mRNAs were also expressed slightly higher level in the gland-like area of cancerous ovaries than that of normal ovaries. F, follicle; NA, nodule area. Scale bar = 100 μm (columns 1-2 and 4-5) and 25 μm (columns 3 and 6).