Figure 5
The purified rERp29 protein was analyzed by Western blot. (A) The purified rERp29 protein (~23 kDa) was confirmed by immunoblotting, using anti-His monoclonal antibody (1:1000 dilution) and rabbit anti-rERp29 IgG purified from sera (1:1000 dilution). (B) ERp29 (~29 kDa) was distinctly detected in BALB/c mouse sperm proteins using rabbit anti-rERp29 IgG (1:1000) but not with IgG from pre-immunized sera. His, anti-His monoclonal antibody; Ab, rabbit anti-rERp29 IgG purified from sera; Pre, IgG from pre-immunized sera.