Figure 2

Western blot analysis of ERp29 in BALB/c mouse epididymal and acrosome-reacted sperm. (A) Lane 1, ERp29 in the whole protein from caput sperm; Lane 3, ERp29 in the whole protein from caudal sperm; Lane 5, ERp29 in the membrane protein from caput sperm; Lane 6, ERp29 in the membrane protein from caudal sperm; Lane 2 and 4, Normal rabbit IgG as negative control; Lanes 7 and 8, β-actin in the whole protein from caput and caudal sperm served as loading control. (B) Lane 1, ERp29 in the whole protein from acrosome intact sperm; Lane 3, ERp29 in the whole protein from acrosome-reacted sperm; Lane 5, ERp29 in the membrane protein from acrosome intact sperm; Lane 6, ERp29 in the membrane protein from acrosome-reacted sperm; Lane 2 and 4, Normal rabbit IgG as negative control; Lane 7 and 8, β-actin in the whole protein from acrosome intact and reacted sperm served as the loading control. (C) Average gray scale levels of the corresponding protein blot in (A) repeated three times. (D) Average gray scale levels of the corresponding protein blot in (B) repeated three times (**P < 0.01).