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Table 6 Relative expression of marmoset MSMB genes.

From: Rapidly evolving marmoset MSMB genes are differently expressed in the male genital tract

   exon 2 and 3 primers exon 3 and 4 primers Average
Tissue Gene 2ΔCT σ % 2ΔCT σ % %
Prostate MSMB1 3.8 × 10-3 1.1 × 10-3 0.4 7.6 × 10-3 1.1 × 10-3 0.7 0.6
Prostate MSMB2 6.9 × 10-3 0.5 × 10-3 0.7 8.5 × 10-3 1.9 × 10-3 0.8 0.8
Prostate MSMB3 1.01 0.17 97.2 1.01 0.11 96.9 97.1
Prostate MSMB4 1.8 × 10-2 0.6 × 10-2 1.8 1.6 × 10-2 0.4 × 10-2 1.6 1.7
Seminal Vesicle MSMB1 4.4 × 10-2 0.8 × 10-2 4.1 7.2 × 10-2 0.8 × 10-2 6.4 5.3
Seminal Vesicle MSMB2 1.1 × 10-2 0.6 × 10-2 1.0 1.6 × 10-2 0.2 × 10-2 1.4 1.2
Seminal Vesicle MSMB3 1.00 0.09 93.5 1.00 0.08 89.0 91.3
Seminal Vesicle MSMB4 1.4 × 10-2 0.6 × 10-2 1.3 3.6 × 10-2 0.4 × 10-2 3.2 2.3
Testis MSMB1 0.34 0.19 15.1 0.62 0.19 25.8 20.5
Testis MSMB2 0.93 0.23 41.5 1.00 0.09 41.8 41.7
Testis MSMB3 0.25 0.09 11.2 0.15 0.07 6.2 8.7
Testis MSMB4 0.72 0.24 32.1 0.63 0.08 26.2 29.2
  1. Common marmoset MSMB were subjected to real-time RT-PCR in the presence of SYBR green. Samples were measured in quadruplicate at different RNA concentrations. Each value is based on a minimal of 8 measurements. The relative amount of transcript is given as 2-ΔCT, with the standard deviation σ. The fraction of MSMB transcripts (given as %) in a tissue was calculated by dividing individual 2-ΔCT values with the sum of 2-ΔCT values of the four MSMB in the tissue.