Rapidly evolving marmoset MSMB genes are differently expressed in the male genital tract

Table 2 Primer pair performance.

Primer pair	slope	R²	Relative efficacy (%)
mspAs1F mspAs1R	-3.42	0.993	98
mspEs1F mspEs1R	-3.36	0.952	99
mspJs1F mspJs1R	-3.29	0.942	100
mspBs1F mspBs1R	-3.32	0.997	100
mspAs2F mspAs2R	-3.36	0.987	99
mspEs2F mspEs2R	-3.32	0.997	100
mspJs2F mspJs2R	-3.30	0.991	100
mspBs2F mspBs2R	-3.34	0.963	100
GAPDHF GAPDHR	-3.31	0.967	100
CTSBF CTSBR	-3.29	0.973	101

Real time PCR was done with serially diluted prostate cDNA. The recovered C_T values were plotted against the logarithms of relative transcript concentrations and analyzed by linear regression. The PCR efficacy (E) was calculated from the equation E = 10^(-1/slope). A perfect doubling of PCR products in each cycle yields a slope of -3.32 and an E-value of 2.00. The relative efficacy is the observed efficacy divided by 2.

ISSN: 1477-7827