Rapidly evolving marmoset MSMB genes are differently expressed in the male genital tract

Table 1 Nucleotide sequences of PCR primers.

Primer name	Primer sequence (5' to 3' orientation)	Gene	Size (bp)
mspAs1F	TCATGCTATTTAATACCCAATAAGATG	MSMB1	140
mspAs1R	ATTTCTTTTTCGAGGCAATCACATTCA	MSMB1
mspEs1F	ATGGATCATGCTATGTAATACGTCATA	MSMB2	160
mspEs1R	AGGGAGCAACATGATATTTCTATGTCA	MSMB2
mspJs1F	CATCATGCTATTTAATACTGAATGACG	MSMB3	149
mspJs1R	ACATGATATTTCTTTTTCTTGGCAAGT	MSMB3
mspBs1F	CATCATGCTATTTAATACTGAATGACA	MSMB4	154
mspBs1R	GTGCAACATGATATTTCTTTTTCGCCA	MSMB4
mspAs2F	AAGGTGGCAGACTGAGAACTGTGATGA	MSMB1	144
mspAs2R	ACCACAATATACTTGCAGTCCTCTTGC	MSMB1
mspEs2F	CTGTGAGCTATGTGCTTGCCGTGACAT	MSMB2	189
mspEs2R	CTAGAAGCACATTACGATATCCATCCA	MSMB2
mspJs2F	AAAGTGGCGGACTGACAGCTGTGACAT	MSMB3	152
mspJs2R	TCTTCTCCACCACAGTTAACTTGCACT	MSMB3
mspBs2F	CTGACAACTGTGAGACATGTGCTTGTG	MSMB4	196
mspBs2R	CTAGAAGCACATTACGATATCCATCCG	MSMB4
GAPDHF	AAAGTGGATGTCGTCGCCATCAATGAT	GAPDH	156
GAPDHR	CTGGAAGATGGTGATGGGATTTCCATT	GAPDH
CTSBF	AGAAGTTCCCCGTGTTCGAGGCTGTGT	CTSB	126
CTSBR	GAGGGAGACTTTGGAATACTCGCAAGT	CTSB

Primers belonging to primer set 1 or 2 contain the designation s1 or s2 in their name respectively. The last letter in the primer names indicate whether the oligonucleotide is priming on the coding (F) or complementary (R) strand. Size, refers to the molecular size of the expected PCR product.

ISSN: 1477-7827