RT-PCR. Transcripts from prostate, seminal vesicles and testis were subjected to RT-PCR, with two different sets of primer pairs, and then analyzed by agarose gel electrophoresis. The ethidium bromide-stained gels are shown. The lettering denotes primer pair specific for MSMB1 (1), MSMB2 (2), MSMB3 (3), MSMB4 (4); negative control (NC) were run with MSMB2 primers on samples where reverse transcriptase were omitted during cDNA synthesis; Mass ruler low molecular size standard (St): there is 0.1 kb between bands and the molecular size of 0.5 kb is indicated by the dense band. The numbers of PCR cycles are given to the right.