ApoD levels in placenta according to pre-pregnancy BMI and GWG. (A) The level of apoD mRNA expression was measured by qRT-PCR using specific primers for apoD. Data are expressed as the normalized ratio of apoD mRNA to 18S RNA (means ± SEM). (B) The placental ApoD protein content was measured by ELISA. The values (means ± SEM) were normalized by the total protein content. * indicates significant differences (p < 0.01) from the normal control group (BMI 20-26 kg/m2, GWG 11-18 kg). # indicates significant differences (p < 0.01) from the group of similar GWG and normal BMI (20-26 kg/m2).