Figure 2

Immunostaining for mPRβ, mPRγ and β-tubulin proteins in the mouse fallopian tube. A: Immunohistochemical (a - f) and immunofluorescent (g - j) detection of mPRβ and β-tubulin, a marker for ciliated epithelial cells. Sections immunolabeled for mPRβ were visualized with MACH 3 rabbit AP (a and d) and green florescence (g). The β-tubulin was stained with MACH 3 mouse HRP (b and e) and red fluorescence (h). The panels' c and f are control sections where the primary antibody was omitted. A double staining was also performed using green and red fluorescent labels for mPRβ and β-tubulin, respectively (i). All fluorescence sections were subsequently counterstained with DAPI to visualized cell nuclei. Confocal microscopy in (j) is showing the overlapping fluorescence of two fluorochromes used. Tubal sections were labelled for mPRβ (green), β-tubulin (red) and merged (yellow). 2B: To facilitate a comparison of the localization of the mPRs, immunostaining for mPRβ (a) mPRγ (b1) β-tubulin (c) and dual staining of mPRγ (green) and β-tubulin (red) (b2) are also presented.