Transcription factors activated transiently either at 30 min or 120 min by PRL treatment in decidua and ovary of PRLR-/-RS mice. Day 9 pseudopregnant PRLR-/-RS mice were injected with ergocryptine (200 μg, sc) for 6 hr followed by a single ip injection of either PRL (60 ug) or vehicle (control). Decidua and ovaries were obtained after 0, 30 and 120 min of PRL treatment. DNA binding activities of transcription factors were measured by Protein/DNA binding assay using nuclear extracts from ovary. (A) Transcription factors activated at 30 min by PRL treatment in the decidua (upper panel) and ovary (lower panel). (B) Transcription factors activated at 120 min by PRL treatment in the decidua (upper panel) and ovary (lower panel).