Animal model and DNA/protein array analysis in the PRLR-/-RS mice. (A) PRLR-/-RS mice were mated with vasectomized males to induce pseudopregnancy. The day the vaginal plug is found, PRLR-/-RS mice were implanted with progesterone pellets. On day 4, decidualization was induced with intrauterine administration of oil. On day 9 of pseudopregnancy, PRLR-/-RS mice were injected with ergocryptine (200 ug, sc) for 6 hr followed by a single ip injection of either PRL (60 ug) or vehicle (control). Ovaries and decidua were obtained after 0, 30 and 120 min of PRL treatment. (B) TranSignal, Protein/DNA array, was performed using nuclear extracts from these tissues. Chart showing percentage of transcription factors activated or inhibited in decidua and ovary.