Modulation of cytokines and transcription factors (T-Bet and GATA3) in CD4 enriched cervical cells of Chlamydia trachomatis infected fertile and infertile women upon stimulation with chlamydial inclusion membrane proteins B and C

Table 1 Details of primers used in this study

No.	Cytokine gene primers	5'-3' sequence
1	GM-CSF F*	AGC CCT GGG AGC ATG TGA
	GM-CSF R**	GAG TAG AGA CAC TGC TGC TGA GAT G
2	IL-β1F	GCT GAT GGC CCT AAA CAG ATG
	IL-β1R	ACGAAT CTC CGA CCA CCA CTA
3	IFNγ F	GAA ACG AGA TGA CTT CGA AAA GCT
	IFNγ R	ATG TCC AAC GCA AAG CAA TAC A
4	IL-12 F	TCG CGT TCA CAA GCT CAA GT
	IL-12 R	CA AAC CTG ACC CAC CCA AGA
5	TNF-α F	GCC CGA CTA TCT CGA CTT TGC
	TNF-α R	A ACC TTC CCA AAC GCC TCC
6	IL4 F	CCA CGG ACA CAA GTG CGA TAT
	IL4 R	CGT AAC AGA CAT CTT TGC TGC C
7	IL5 F	AAA GGC AAA CGC AGA ACG TT
	IL5 R	CTC TTG GAG CTG CCT ACG TGT
8	IL6 F	ACT CAC CTC TTC AGA ACG
	IL6 R	AGT AGT GAG GAA CAA GCC
9	IL10 F	GGG AGA ACC TGA AGA CCC TCA
	IL10 R	AAC AAG AGC AAG GCC GTG G
10	T-Bet(TBX21) F	AAC ACA GGA GCG CAC TGG AT
	T-Bet(TBX21) R	ATT GTG CTC CAG TCC CTC CA
11	GATA3 F	AGA TGG CAC GGG ACA CTA CCT
	GATA3 R	ATT AAG CCC AAG CGA AGG C
12	B-actin F	TGT GAT GGT GGG TAT GGG TC
	B-actin R	TAC AAT GAG CTG CGT GT

ISSN: 1477-7827