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Figure 1 | Reproductive Biology and Endocrinology

Figure 1

From: Isolation and characterization of bovine herpesvirus 4 (BoHV-4) from a cow affected by post partum metritis and cloning of the genome as a bacterial artificial chromosome

Figure 1

A) Representative microscopic (10×) IFAT images of sera from the cow which was used to isolate BoHV-4-U. The presence of anti-BoHV-4 antibodies in the first and second sample (6 months following the first) are detectable by green cells when observed with a FITC filter and the negative controls appears brown due to the Evans blue dye. Counterstaining with Evans blue dye observed with a TRITC/filter (red pictures) was used to monitor the integrity of the cell substrate. The merged pictures (FITC/TRITC) allow co-localization of the anti-BoHV-4 antibody staining with the cellular substrate. B) Representative phase contrast microscopic images (10×) of BoHV-4-U cytopathic effect generating plaques. C) Representative phase contrast microscopic images (10×) of BoHV-4-U plaques immuno-peroxidase stained with anti-BoHV-4 antibodies.

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