Real-time quantitative PCR analysis of A) Tac1 ; B) Tac2 and C) Tac4 expression in uterine cDNA from mice treated with E
, alone or in combination. Uteri were collected from ovariectomized mice untreated (control) or treated with E2 (1 μg/mouse, 72 h) or its vehicle, P4 (2 mg/mouse per day for 2 days) or its vehicle, and E2 + P4 or the corresponding vehicles. Values are expressed as the fold change of each target-gene expression, relative to the geometric mean mRNA expression of 4 housekeeping genes. Each bar represents the mean of uterine cDNA samples from 5–10 different mice, with S.E.M. shown by vertical lines. aP < 0.05, E2 vs. E2 vehicle, unpaired t test; bP < 0.05, P4 vs. P4 vehicle, unpaired t test; cP < 0.05, E2 + P4, E2 + P4 vehicle and E2 vehicle + P4 vs. E2 vehicle + P4 vehicle, one-way ANOVA.