Real-time quantitative PCR analysis of A) Tac1 ; B) Tac2 and C) Tac4 expression in uterine cDNA from mice treated with E
, propylpyrazole triol (PPT), diarylpropionitrile (DPN)or estren. Uteri were collected from ovariectomized mice untreated (control) or treated for 3 or 24 h with E2 (1 μg/mouse) PPT (75 μg/mouse) DPN (100 μg/mouse) estren (300 μg/mouse) or the corresponding vehicle. Values are expressed as the fold change of each target-gene expression, relative to the geometric mean mRNA expression of 4 housekeeping genes. Each bar represents the mean of uterine cDNA samples from 5–10 different mice, with S.E.M. shown by vertical lines. *P < 0.05, significant difference versus mRNA levels in uteri from ovariectomized mice treated for 3 h with vehicle; ♦P < 0.05, significant difference versus mRNA levels in uteri from ovariectomized mice treated for 24 h with vehicle; one-way ANOVA.