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Table 1 Oligonucleotide Primers and Amplification Conditions for RT-PCR

From: Characterization of a novel telomerase-immortalized human endometrial stromal cell line, St-T1b

Amplified Product Forward/Reverse Primer Amplification Conditions Product Size Genbank Accession No.
DEPP
(C10orf10)
5'-AGGTCCCGGCTTCTGCTCTCC-3'
5'-CCCATGGGCTTGCTGCTGTC-3'
20 cycles
95°C 30 sec, 60°C 30 sec, 72°C 45 sec
434 bp NM_007021
dPRL
(decidua-specific PRL transcript)
5'-GAGACACCAAGAAGAATCGGAACATACAGG-3'
5'-TCGGGGGTGGCAAGGGAAGAA-3'
25 or 30 cycles
94°C 45 sec, 57°C 45 sec, 72°C 45 sec
416 bp M58594,
GQ305133
ERα
(ESR1)
5'-CCCGCCGGCATTCTACA-3'
5'-ACATTCTCCCTCCTCTTCGGTCTT-3'
30 cycles
95°C 30 sec, 52°C 30 sec, 72°C 45 sec
359 bp NM_000125
GAPDH 5'-GGAGTCCACTGGCGTCTTCAC-3'
5'-GAGGGGCCATCCACAGTCTTCT-3'
20 cycles
95°C 30 sec, 65°C 30 sec, 72°C 30 sec
287 bp NM_002046
HEF-1
(NEDD9)
5'-AACCGGGTGAAGCTTCTGATTGG-3'
5'-GCTGATGAGGGAGGGATGTCGTAT-3'
25 cycles
95°C 45 sec, 62°C 45 sec, 72°C 60 sec
416 bp NM_006403
IGFBP-1 5'-TGCTGCAGAGGCAGGGAGCCC-3'
5'-AAGGATCCTCTTCCCATTCCA-3'
25 cycles
95°C 30 sec, 63°C 30 sec, 72°C 90 sec
378 bp NM_000596
ITGA6 5'-AGCAAGGCAGATGGAATAATGTGA-3'
5'-AGGCCGGGATCTGAAAATAGTT-3'
25 cycles
95°C 45 sec, 55°C 45 sec, 72°C 60 sec
329 bp NM_000210
KAI-1
(CD82)
5'-AGAAGTGGGCCCTGTGACC-3'
5'-TTGCCCATGTTGAAGTAGAAGAG-3'
25 cycles
95°C 30 sec, 58°C 30 sec, 72°C 90 sec
396 bp NM_002231
p53
(TP53)
5'-TTCCACGACGGTGACACGCTT-3'
5'-GTAGCTGCCCTGGTAGGTTTTCTG-3'
25 cycles
95°C 30 sec, 57°C 30 sec, 72°C 45 sec
381 bp NM_000546
PR-B
(PGR)
5'-GCCACATTCAACACCCACTTTCTC-3'
5'-GCCTCCAGCACCCCTTGTAGC-3'
30 cycles
95°C 45 sec, 52°C 45 sec, 72°C 60 sec
411 bp NM_000926
PR-B/-A 5'-TCCACGTGCCTATCCTGCCTCTCA-3'
5'-GCCGTCACCGCCGCTTCC-3'
30 cycles
95°C 45 sec, 61°C 45 sec, 72°C 60 sec
412 bp NM_000926
PR-LBD 5'-GGACATGACAACACAAAACCTGAC-3'
5'-GTGCCCGGGACTGGATAAAT-3'
30 cycles
95°C 45 sec, 52°C 45 sec, 72°C 60 sec
598 bp NM_000926
Tissue Factor
(F3)
5'-TACTTGGCACGGGTCTTCTCCTAC-3'
5'-TTCTCCTGGCCCATACACTCTACC-3'
20 cycles
95°C 30 sec, 60°C 30 sec, 72°C 45 sec
431 bp NM_001993