Table 1 Oligonucleotide Primers and Amplification Conditions for RT-PCR

DEPP

(C10orf10)

5'-AGGTCCCGGCTTCTGCTCTCC-3'

5'-CCCATGGGCTTGCTGCTGTC-3'

20 cycles

95°C 30 sec, 60°C 30 sec, 72°C 45 sec

434 bp

NM_007021

dPRL

(decidua-specific PRL transcript)

5'-GAGACACCAAGAAGAATCGGAACATACAGG-3'

5'-TCGGGGGTGGCAAGGGAAGAA-3'

25 or 30 cycles

94°C 45 sec, 57°C 45 sec, 72°C 45 sec

416 bp

M58594,

GQ305133

ERα

(ESR1)

5'-CCCGCCGGCATTCTACA-3'

5'-ACATTCTCCCTCCTCTTCGGTCTT-3'

30 cycles

95°C 30 sec, 52°C 30 sec, 72°C 45 sec

359 bp

NM_000125

GAPDH

5'-GGAGTCCACTGGCGTCTTCAC-3'

5'-GAGGGGCCATCCACAGTCTTCT-3'

20 cycles

95°C 30 sec, 65°C 30 sec, 72°C 30 sec

287 bp

NM_002046

HEF-1

(NEDD9)

5'-AACCGGGTGAAGCTTCTGATTGG-3'

5'-GCTGATGAGGGAGGGATGTCGTAT-3'

25 cycles

95°C 45 sec, 62°C 45 sec, 72°C 60 sec

416 bp

NM_006403

IGFBP-1

5'-TGCTGCAGAGGCAGGGAGCCC-3'

5'-AAGGATCCTCTTCCCATTCCA-3'

25 cycles

95°C 30 sec, 63°C 30 sec, 72°C 90 sec

378 bp

NM_000596

ITGA6

5'-AGCAAGGCAGATGGAATAATGTGA-3'

5'-AGGCCGGGATCTGAAAATAGTT-3'

25 cycles

95°C 45 sec, 55°C 45 sec, 72°C 60 sec

329 bp

NM_000210

KAI-1

(CD82)

5'-AGAAGTGGGCCCTGTGACC-3'

5'-TTGCCCATGTTGAAGTAGAAGAG-3'

25 cycles

95°C 30 sec, 58°C 30 sec, 72°C 90 sec

396 bp

NM_002231

p53

(TP53)

5'-TTCCACGACGGTGACACGCTT-3'

5'-GTAGCTGCCCTGGTAGGTTTTCTG-3'

25 cycles

95°C 30 sec, 57°C 30 sec, 72°C 45 sec

381 bp

NM_000546

PR-B

(PGR)

5'-GCCACATTCAACACCCACTTTCTC-3'

5'-GCCTCCAGCACCCCTTGTAGC-3'

30 cycles

95°C 45 sec, 52°C 45 sec, 72°C 60 sec

411 bp

NM_000926

PR-B/-A

5'-TCCACGTGCCTATCCTGCCTCTCA-3'

5'-GCCGTCACCGCCGCTTCC-3'

30 cycles

95°C 45 sec, 61°C 45 sec, 72°C 60 sec

412 bp

NM_000926

PR-LBD

5'-GGACATGACAACACAAAACCTGAC-3'

5'-GTGCCCGGGACTGGATAAAT-3'

30 cycles

95°C 45 sec, 52°C 45 sec, 72°C 60 sec

598 bp

NM_000926

Tissue Factor

(F3)

5'-TACTTGGCACGGGTCTTCTCCTAC-3'

5'-TTCTCCTGGCCCATACACTCTACC-3'

20 cycles

95°C 30 sec, 60°C 30 sec, 72°C 45 sec

431 bp

NM_001993