Figure 1

The JEG3 cells were transiently transfected overnight with AP-1 luciferase and β-galactosidase expression vectors. The cells were stimulated with cAMP for 5 hr and AP-1 promoter activation was assessed by performing luciferase assay. Calorimetric β-galactosidase assay was performed to correct for the transfection efficiency. Luciferase activity was expressed as relative light unit (RLU). (*p < 0.01 compared to media treated cells). Each experiment was performed in triplicate or quadruplicate and repeated at least three independent times.