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Figure 6 | Reproductive Biology and Endocrinology

Figure 6

From: Seminiferous tubule transfection in vitro to define post-meiotic gene regulation

Figure 6

A-B. Cryosections showing EYFP fluorescence in almost all cell types 24 h following microinjection and electroporation of the CMV-driven EYFP-N1 plasmid. Note that only one half of the tubule is successfully electroporated. The tubule outline is shown by the white dashed line. C-F. EYFP fluorescence in different cell types 24 h following microinjection and electroporation of the CMV-driven EYFP-N1 with subsequent dissolution of the tubule and cytospin centrifugation of individual cells. Cell nuclei are labelled using TO-PRO. G-H. Direct fluorescence of EYFP in intact seminiferous tubules 24 h following microinjection and electroporation of the CMV-driven EYFP-N1 plasmid.

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