Figure 4
From: Seminiferous tubule transfection in vitro to define post-meiotic gene regulation
A. A comparison of the transfection efficiency for the control plasmid EYFP-N1 of different stages of spermatogenesis (judged by transillumination) after 24 h incubation. Each row represents typical individual tubules for each of the indicated stages. B. A similar comparison but using the reporter plasmid pGL3-C, expressing the luciferase gene, and quantifying the specific luminescence after 24 h incubation from 10 tubules of each stage. For the control, tubules were transfected with the promoterless control plasmid pGL3-B, in order to determine any background endogenous luminescence.