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Figure 4 | Reproductive Biology and Endocrinology

Figure 4

From: Activin A induces ovine follicle stimulating hormone beta using -169/-58 bp of its promoter and a simple TATA box

Figure 4

Replacement mutations between -4741/+701 bp of ovine FSHBLuc. Ovine FSHBLuc was divided into 9 sections to determine where activin-responsive sequences might be located. For replacement constructs 1–5, deleted regions are designated with a box that indicates what sequences were substituted for the deletion. For replacement constructs 6–9, the section deleted and replaced is written above the promoter line. The replacement sequences for 6–9 are identified in Materials and Methods. Fold-induction was calculated as a ratio of luciferase expression ± activin A corrected for Renilla co-expression. This Luciferase/Renilla ratio was 54 for WT induction. Basal expression was calculated the same way and normalized to wild type basal expression (WT = 1). The ratio of Luciferase/Renilla was 10 for WT basal expression. Bars represent the mean ± sem of results from quadruplicate cultures from a single but representative complete experiment. Means with different letters are statistically different from wild type induction (fold-induction). Means with different symbols are statistically different from wild type basal expression (relative basal expression). ANOVA and Student Newman-Keuls analyses were used to determine significance between means using P ≤ 0.05.

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