Hormone-regulated expression and distribution of versican in mouse uterine tissues

Table 1 Mouse oligonucleotide primers (5'→3')

Gene	Forward (F) and Reverse (R) primers	Amp. size	Genbank #
*GAPDH	F:⁸⁴⁸TCTGAGGGCCCACTGAAG⁸⁶⁵; R:¹⁰⁴⁷AGGGTTTCTTACTCCTTGGAGG¹⁰⁶⁸	221 bp	NM_001001303
*Versican	F:⁹⁵³²TCCTGATTGGCATTAGTGAAG ⁹⁵⁵²; R:⁹⁶⁷²CTGGTCTCCGCTGTATCC ⁹⁶⁸⁹	158 bp	NM_001081249
V0	F:³⁷⁵⁹TTCACAGAACGCCACCCTTGAGTCC³⁷⁸³; R:⁴³⁶³CTAGCTTCTGCAGCTTCCGGGTCC ⁴³⁸⁶	628 bp	NM_28599
V1	F:¹⁰³¹GCAGCTTGGAGAAATGGCTTTGACC¹⁰⁵⁵; R:⁴³⁶³CTAGCTTCTGCAGCTTCCGGGTCC ⁴³⁸⁶	443 bp	NM_28599
V2	F:³⁸⁵⁶TCCTGGAGAATCTGTAACACAGCACCC³⁸⁸²; R:⁹³⁶⁴CTCGGTAGGATAACAGGTGCCTCCG⁹³⁸⁸	307 bp	NM_28599
V3	F:¹⁰²⁴ACTTCAGGCAGCTTGGAGAAATGGC¹⁰⁴⁸; R:⁹⁴¹⁰ACTGGTCTCCGCTGTATCCAGGTGC⁹⁴³⁴	302 bp	NM_28599

V0, V1, V2 and V3 are alternative splicing variants of the versican gene. *primers used for real-time PCR analysis.

ISSN: 1477-7827