Expression of genes associated with immunity in the endometrium of cattle with disparate postpartum uterine disease and fertility

Table 1 Oligonucleotide primer sequences.

Gene		Primer sequence	Size	Tm (°C)	Accession No.
CD45	Sense	CTCGATGTTAAGCGAGAGGAAT	185	56	AJ400864
	Anti sense	TCTTCATCTTCCACGCAGTCTA
CD14	Sense	GGGTACTCTCTGCTCAAGGAAC	199	56	NM_174008
	Anti sense	CTTGGGCAATGTTCAGCAC
MD-2	Sense	GGGAAGCCGTGGAATACTCTAT	204	54	DQ319076
	Anti sense	CCCCTGAAGGAGAATTGTATTG
NOD1	Sense	GTCACTCACATCCGAAACACTC	213	55	XM_598513
	Anti sense	CCTGAGATCCACATAAGCGTCT
IL1A	Sense	AGAGGATTCTCAGCTTCCTGTG	224	54	NM_174092
	Anti sense	ATTTTTCTTGCTTTGTGGCAAT
IL1B	Sense	GAGGAGCATCCTTTCATTCATC	229	56	X54796
	Anti sense	TTCCTCTCCTTGTACGAAGCTC
IL1R2	Sense	ATCCCATGTAAGGTGTTTCTGG	181	56	AB219098
	Anti sense	TGACAGGATCAAAAATCAGTGG
TNF	Sense	ACTCAGGTCCTCTTCTCAAGCC	774	56	NM_173966
	Anti sense	ATGATCCCAAAGTAGACCTGCC
IL6	Sense	ATGACTTCTGCTTTCCCTACCC	180	56	NM_173923
	Anti sense	GCTGCTTTCACACTCATCATTC
IFN-a	Sense	AGAGCCTCCTGGACAAGCTAC	212	56	NM_001017411
	Anti sense	CATGACTTCTGCTCTGACAACC
NOS2	Sense	GGACAGTAAAGACGTCTCCAGA	197	54	AF340236
	Anti sense	TATGGTCAAACTTTTGGGGTTC
IL10	Sense	TACTCTGTTGCCTGGTCTTCCT	178	56	NM_174088
	Anti sense	AGTAAGCTGTGCAGTTGGTCCT
TGFBR1	Sense	CAGGTTTACCATTGCTTGTTCA	243	56	NM_174621
	Anti sense	TGCCATTGTCTTTATTGTCTGC
PTGER2	Sense	GTTCCACGTGTTGGTGACAG	246	56	AF539402
	Anti sense	ACTCGGCGCTGGTAGAAGTA
PTGER4	Sense	TCGTGGTGCTCTGTAAATCG	226	56	AF539403
	Anti sense	CTCATCGCACAGATGATGCT

Oligonucleotide primer sequences, expected amplicon size and annealing temperatures used in real-time PCR assays.

ISSN: 1477-7827