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Table 1 Antisera utilized for immunofluorescence and immunoblot analysis.

From: Integrin-linked kinase can facilitate syncytialization and hormonal differentiation of the human trophoblast-derived BeWo cell line

Antisera

Method

Dilution

Company

Catalogue #

Mouse anti-ILK; Clone 65.1.9

IF

1:100

Abcam, Ltd, Cambridge, MA, USA

ab49979

Rabbit anti-ILK

IB

1:2000

Cell Signaling Technology, Beverly, MA, USA

3862

Rabbit anti-E-cadherin

IF

1:250

Abcam, Ltd, Cambridge, MA, USA

ab15148

Mouse anti-E-cadherin, clone SHE78-7

IF

IB

1:1000

1:10000

EMD Biosciences, San Diego, CA, USA

205602

Rabbit anti-hCG

IF

1:500

Dako Canada, Inc, Mississauga, ON, Canada

A0231

Rabbit anti-Snail

IB

1:1000

Abcam, Ltd, Cambridge, MA, USA

ab17732

Mouse anti-pAKT1 (Ser 473); Clone 587F11

IF

1:100

Cell Signaling Technology, Beverly, MA, USA

4051

Rabbit anti-pAKT1 (Ser 473)

IB

1:1000

Cell Signaling Technology, Beverly, MA, USA

9271

Anti-phospho-GSK3β (Ser-9)

IB

1:1000

Cell Signaling Technology, Beverly, MA, USA

9336

FITC-Sheep anti-Rabbit IgG

IF

1:250

Sigma Chemical Co, St. Louis, MO, USA

F7512

RRX-Donkey anti-Mouse IgG

IF

1:150

Jackson ImmunoResearch Labs Inc, West Grove, USA

715-295-150

RRX-Donkey anti-Rabbit IgG

IF

1:250

Jackson ImmunoResearch Labs Inc, West Grove, USA

711-295-152

ChromPure Mouse IgG

IF

N/A*

Jackson ImmunoResearch Labs Inc, West Grove, USA

015-000-003

ChromPure Rabbit IgG

IF

N/A*

Jackson ImmunoResearch Labs Inc, West Grove, USA

011-000-003

HRP-Goat anti-Rabbit IgG (H+L)

IB

1:20000

Pierce, Rockford, IL, USA

31460

HRP-Goat anti-Mouse IgG (H+L)

IB

1:10000

Pierce, Rockford, IL, USA

31430

  1. IF: immunofluorescence, IB: immunoblot, FITC: Fluorescein isothiocyanate, RRX: Rhodamine-Red-X, HRP: horseradish peroxidase, *Dependent on concentration of primary antisera utilized