Figure 7

ILK, pAkt, and Snail protein expression during a timecourse of BeWo syncytialization. A) Representative immunoblots from four independent experiments (n = 4) are shown with a representative immunoblot stained for total protein to illustrate comparable protein loading of all gel lanes. B) Densitometric analysis of pAkt expression during BeWo cell culture in syncytialization conditions demonstrating a significant decrease in pAkt expression by 24 h (p < 0.05) compared to 0, 9, 12 and 48 h and a rapid increase in pAkt expression by 48 h of culture compared to 0 h (p < 0.05, n = 4). C) Densitometric analysis of Snail protein expression during BeWo cell culture in syncytialization conditions demonstrating that Snail expression becomes significantly elevated at 24, 36 and 48 h vs 12 h (p < 0.05, n = 4) and at 48 h of culture compared to expression in proliferating conditions at 0 h (p < 0.05, n = 4).