Figure 3

Immunofluorescence analyses for E-cadherin (left panels) and ILK (right panels) protein expression in BeWo cells at 12 h and 24 h of cell culture in syncytialization conditions. The images demonstrate that ILK localization to cell nuclei correlates with downregulation of E-cadherin expression at cell-cell membranes. IgG insets, non-specific immunoglobulins of the appropriate animal species were used as specificity controls in place of the appropriate primary antisera. Nuclei in E-cadherin immunofluorescence panels were stained with DAPI. Scale bars = 25 μm.