Figure 2

Immunofluorescence analyses for E-cadherin (left panels) and ILK (right panels) protein expression in BeWo cells during a timecourse of syncytialization. Cells were cultivated under proliferating conditions (0 h) or syncytialization conditions (12 h – 48 h). With increasing culture time in syncytialization conditions, ILK began to localize to cell nuclei in addition to the cytoplasm and to focal adhesions (arrowheads, 24 h – 48 h). Arrows in 0 h panels show the position of apparent ILK and E-cadherin co-localization demonstrated in the insets. IgG insets, non-specific IgGs of the appropriate animal species were used as specificity controls in place of the appropriate primary antisera. Nuclei in E-cadherin immunofluorescence panels were stained with DAPI. Scale bars = 25 μm.