Localization of placental TRPV6 at P20.5. Left panel, overview of placenta divided into three distinct areas (A to C) showing TRPV6 immuno-staining as spotted rectangles. A, inner labyrinth area; B, middle labyrinth; C, spongy zone. 'No first antibody' indicates immunoreactivity without anti-TRPV6 treatment, as a negative control. Arrows indicate immuno-positive staining.