Figure 2
From: The estrogen-injected female mouse: new insight into the etiology of PCOS
E 2 - and T-induced changes in thymocyte levels of female B6A mice. Animals in (A) were given 3 daily sc injections of 10 μl sesame oil:ethanol (9:1; v:v) [vehicle] with or without 20 μg E2, starting at 5 days of age. They were killed at 8, 9, 10, 11, and 12 days of age. Each data-point consists of the average number of viable thymocytes isolated from thymuses of 2 animals. In (B) the animals were given 4 daily injections of either 10 μl vehicle, or vehicle containing 20 μg T, starting at 0 days of age. They were killed at 5, 7, 9, and 12 days of age. Five animals were used for each data-point. Thymocytes were visualized using the Trypan Blue Exclusion Test and counted in a hemocytometer.