Figure 8From: Characterization of antibodies for quantitative determination of spiggin protein levels in male and female three-spined stickleback (Gasterosteus aculeatus)Spiggin γ expression in cell and tissue lysates was examined by Western blot. A. Detection of recombinant spiggin γ protein using the polyclonal native spiggin antibody; B. detection of His-tagged proteins using anti-His-tag antibody. MW – molecular weight markers (kDa); Control – bacterial lysate prior to IPTG addition; Expressed – bacterial supernatant following IPTG addition. The arrows indicate the position of spiggin γ protein.Back to article page