Figure 6
From: Reduced expression of CRH receptor type 1 in upper segment human myometrium during labour
PCR analysis of the human CRH-R1 variants in myometrium. (A) CRH-R1 has eight different receptor isoforms (modification based on the scheme from ref 22). The representative results show the nested PCR products of CRH-R1in which (B) the primers that go across exon 2–7 were used, and (C) the primers that go across exon 9–14. Variants are distinguished by different molecular weight of amplified bands. Bands indicated by arrow 1 can only be CRH-R1β; arrow 2 can be CRH-R1α, -R1c, -R1f, or -R1g isoforms; arrow 3 are specific for isoform CRH-R1c; arrow 4 can be CRH-R1e. Bands indicated with arrow 5 can be CRH-R1α, -R1β, or -R1c isoforms; arrow 6 indicates CRH-R1f. arrow 7 indicates CRH-R1g. NP: nonpregnant group; TNL: term nolabour group; TL: term labour group; M: molecular weight marker. Lane 1,3,5: LS samples. Lane 2,4,6: US samples.