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Figure 7 | Reproductive Biology and Endocrinology

Figure 7

From: Mechanisms of matrix metalloproteinase-2 (mmp-2) transcriptional repression by progesterone in jar choriocarcinoma cells

Figure 7

Progesterone reduces SP4 through enhanced degradation and ubiquitin binding rather that inhibition of protein synthesis. (A) Immunofluorescence staining of JAR cells for SP4 protein in the presence or absence of progesterone and cycloheximide (CHX). Red staining represents SP4 protein. (B) Western blot analysis for Ubiquitin binding to the corresponding SP protein of immunoprecipated SP1-4 and PR proteins from cells cultured for four hours in the presence or absence of progesterone (1 μM). Treatment with progesterone specifically promotes Ubiquitin binding to the SP4 protein.

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