Figure 6

Chromatin immunoprecipitation analysis of SP1-4 and PR interactions with the progesterone regulatory region within a genomic context. Progesterone reduces JAR nuclear SP4 concentration. (A) Chromatin immunoprecipitation was performed as detailed in Experimental Procedures using sheared chromatin prepared from JAR cells after four hours incubation in the presence or absence of progesterone (1 μM). Treatment with progesterone results in decreased binding of SP4 and PR to the progesterone regulatory region of the MMP-2 promoter. (B) Western blots of JAR cell nuclear extracts from cells after four hours incubation in the presence or absence of progesterone (1 μM). Progesterone treatment specifically reduces JAR cell nuclear SP4 levels.