Figure 2

Progesterone suppresses JAR MMP-2 secretion and synthesis. (A) Secretion of pro-MMP-2 by JAR cells after 48 hours incubation in the absence or presence of progesterone alone (1 μM–10 μM) or in combination with Mifepristone (MI; 10^-6 M). Results represent mean ± SEM from 4 independent experiments *P < 0.05 versus untreated cells. Total RNA of MMP-2 isolated form JAR cells treated with progesterone (1 μM) for the indicated times was assessed by Northern blot (B) and semiquantitative reverse transcription-PCR (C). The data are presented as the ratio of MMP-2 to GAPDH. The values represent the mean ± SEM from 4 different experiments performed in duplicates.