Figure 4

Effect of LH on intracellular glucocorticoid concentration. Leydig cells were transfected transiently with a GRE-Luc reporter plasmid, and intracellular glucocorticoid concentration was estimated by GR-mediated transcription of GRE-Luc. Leydig cells were treated with CORT (50 nM), LH (20 ng/mL) or CORT plus LH as described in Methods. Dissimilar superscripts indicate significant differences between groups (P < 0.05).